Mapping of Two New Rust Resistance Genes Uvf-2 and Uvf-3 in Faba Bean

Faba bean is gaining attention in Australia as a rotation grain legume where most of the country’s produce exported. Rust, caused by Uromyces viciae-fabae, major constraint to production eastern and its chemical control results increased cost production. The deployment diverse sources resistance new cultivars underpins economic eco-friendly rust diseases crops. A selection from cultivar Doza (Doza#12034) European accession Ac1655 exhibited seedling against U. viciae-fabae pathotype 24–40. Doza#12034 were crossed with susceptible genotype Fiord recombinant inbred line (RIL) F6 populations generated. Rust tests on Fiord/Doza#12034 Fiord/Ac1655 F4 demonstrated monogenic inheritance both crosses underlying loci named Uvf-2 Uvf-3, respectively. Genetic mapping RIL located Uvf-3 chromosomes III V, SNPs that showed linkage converted into kompititive allele specific PCR (KASP) assays. Markers KASP_Vf_0703 KASP_C250539 flanked at 4.9 cM 2.9 distances, whereas was KASP_Ac×F165 (2.5 cM) KASP_vf_1090 (10.1 cM). can be used for marker-assisted respectively, after confirming parental polymorphisms.